Aggregation Number Calculator

Estimate micelle size using molecular weight or concentration. Validate inputs, track runs, and compare scenarios. Download CSV and PDF summaries for your notebook easily.

Calculator

Choose a method, enter values, then submit.

Tip: Use the concentration method when you have CMC and micelle counts.
Aggregate molecular weight from light scattering or SEC.
Single surfactant or monomer molecular weight.
Interpretation
Values near 50–150 are common for many micelles, but depend on chemistry and conditions.
Total monomers counted or estimated in the system.
Number of aggregates, micelles, or clusters.
Sanity check
If aggregates are larger, Nagg should increase proportionally.
Total surfactant concentration in solution.
Critical micelle concentration under your conditions.
Total sample volume used for counting micelles.
Micelle count from scattering, imaging, or model fit.
Constant used
NA = 6.022141e+23 molecules/mol.
Requirement
Ctotal must exceed CMC to form micelles.
Results appear below the header after submission. Downloads use your current table.

Example data table

These sample cases show typical inputs and computed aggregation numbers.
Scenario Method Key inputs Nagg
Micelle by Mw Molecular-weight ratio Mw(agg)=57,600 g/mol; Mw(mono)=288 g/mol 200
Cluster counts Count ratio Total molecules=2.40×1018; Aggregates=2.00×1016 120
Above CMC Concentration-to-count Ctotal=0.050; CMC=0.008; V=0.250 L; Micelles=1.50×1020 42.2

Calculation history

Up to 20 recent runs are stored for this browser session.
Time Method Nagg Inputs
No history yet. Submit a calculation to start tracking runs.

Why aggregation number matters in solution chemistry

Aggregation number (Nagg) links microscopic structure to macroscopic behavior. In micellar systems, it influences solubilization capacity, viscosity trends, and interfacial activity. A higher Nagg typically indicates larger assemblies, which can shift light scattering intensity and alter diffusion. Tracking Nagg across temperature, ionic strength, and co-solvent fraction helps explain phase boundaries and stability windows in formulation work.

Choosing the right measurement pathway

The calculator supports three pathways that map to common lab data streams. The molecular-weight ratio approach fits datasets from static light scattering, SEC-MALS, or calibrated mass measurements. The count ratio method is useful when microscopy, particle counting, or simulation outputs provide aggregate counts directly. The concentration-to-count approach matches studies where CMC is known and aggregate number density can be estimated from scattering models.

Input quality controls that reduce lab errors

Aggregation estimates are sensitive to unit consistency and baseline assumptions. Concentrations must be in mol/L and volume in liters for the Avogadro conversion. For the micelle method, Ctotal must exceed CMC; otherwise, micellized material is effectively zero. Using scientific notation for large counts prevents rounding errors when values exceed 1012. The step summary helps confirm each intermediate quantity before reporting.

Interpreting results across experiments

Nagg is not a universal constant; it shifts with surfactant type, counterions, additives, and concentration. When comparing runs, keep temperature and ionic strength fixed, then vary one factor at a time. If Nagg rises while CMC falls, stronger hydrophobic association is a plausible driver. If Nagg falls with added salt, electrostatic screening may be changing curvature or promoting smaller aggregates depending on headgroup chemistry.

Reporting and exporting for documentation

The history table stores recent calculations so you can compare scenarios in one session. Exporting CSV supports quick import into spreadsheets for plotting Nagg versus concentration or temperature. The PDF export captures the same table for lab notebooks, QA packets, or client deliverables. Together, these outputs standardize reporting and make it easier to reproduce calculations during audits and peer review. Add notes on sample ID, buffer, and instrument settings to keep your aggregation datasets comparable. Consistent metadata improves traceability and reduces recalculation when methods change later unexpectedly.

FAQs

What is an aggregation number?

It is the average number of monomers or surfactant molecules contained in one aggregate, such as a micelle, vesicle, or cluster, under specified conditions.

Which method should I use first?

Start with the molecular-weight ratio if you have reliable Mw values. Use count ratio when you have particle counts. Use the concentration method when CMC is known and you can estimate micelle number density.

Why must Ctotal be greater than CMC?

Below CMC, most surfactant remains as monomers, so the micellized fraction is negligible. The concentration method assumes only the excess over CMC contributes to micelles.

Can I enter scientific notation?

Yes. Inputs accept formats like 1.5e20 for large counts. This helps keep precision when working with Avogadro-scale quantities or model-derived number densities.

Are results always integers?

Not necessarily. Real systems show distributions of aggregate sizes, so Nagg is often a non-integer average. Rounding for reporting is acceptable, but keep raw values for comparisons.

What should I report with Nagg?

Report temperature, ionic strength, solvent composition, concentration, and measurement technique. These factors strongly influence aggregation and help others reproduce or compare your results.

Formula used

Notes: Keep units consistent. Concentrations are in mol/L and volume in liters.

How to use this calculator

  1. Pick a method that matches your measurements.
  2. Enter values using scientific notation if needed (e.g., 1.5e20).
  3. Press Submit to display results under the header.
  4. Review the input list and step summary for quick validation.
  5. Use the download buttons to export your current history table.

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Important Note: All the Calculators listed in this site are for educational purpose only and we do not guarentee the accuracy of results. Please do consult with other sources as well.