Calculator Inputs
Saved Result History
| # | Mode | Main Result | Value | Unit | Timestamp |
|---|---|---|---|---|---|
| No saved results yet. | |||||
CSV export includes saved history. PDF export captures the current result block and graph.
Formula Used
Corrected concentration
Ccorr = C × (Purity / 100) × (Recovery / 100) ÷ Dilution Factor
Normalized peak area
Anorm = A × (Target Injection Volume ÷ Actual Injection Volume)
Absolute response factor
RF = Anorm ÷ Ccorr
Relative response factor
RRF = (Aanalyte,norm ÷ Canalyte,corr) ÷ (Areference,norm ÷ Creference,corr)
Unknown corrected concentration from a known response factor
Cunknown,corr = Aunknown,norm ÷ RF
Unknown corrected concentration from a known relative response factor
Cunknown,corr = (Aunknown,norm ÷ Areference,norm) × (Creference,corr ÷ RRF)
Recovered stock concentration
Cstock = Ccorr × Dilution Factor ÷ [(Purity / 100) × (Recovery / 100)]
These equations are useful for GC, HPLC, and similar detector response comparisons when you must account for purity, dilution, and injection normalization.
How to Use This Calculator
- Choose the calculation mode that matches your lab task.
- Set the concentration unit label, such as mg/L or µg/mL.
- Enter the normalization injection volume used for comparing runs.
- Fill in peak area, concentration, purity, recovery, dilution, and actual injection volume.
- Submit the form to show the result above the form.
- Review the detailed values, formula summary, and Plotly graph.
- Export the current result to PDF or save history to CSV.
- Use the example button to instantly test each mode with sample values.
Example Data Table
| Run | Peak Area | Stock Conc. (mg/L) | Purity (%) | Dilution | Injection (µL) | Corrected Conc. (mg/L) | Normalized Area |
|---|---|---|---|---|---|---|---|
| Standard 1 | 125000 | 50.00 | 99.5 | 1.00 | 1.00 | 49.75 | 125000 |
| Standard 2 | 100200 | 40.00 | 99.0 | 1.00 | 1.00 | 39.60 | 100200 |
| Analyte Mix | 94500 | 30.00 | 98.5 | 1.00 | 1.00 | 29.55 | 94500 |
| Unknown | 78200 | — | 100.0 | 2.00 | 1.00 | Calculated | 78200 |
You can compare your own runs against these values to verify the calculator workflow.
FAQs
1) What is a response factor in chemistry?
A response factor links detector signal to analyte concentration. It helps convert peak area into concentration when detector sensitivity is stable for the same method conditions.
2) When should I use relative response factor instead?
Use relative response factor when an analyte is compared against a reference or internal standard. This is helpful when direct calibration is limited or when the method uses a standard-response relationship.
3) Why does the calculator ask for purity?
Purity adjusts the stock concentration to the actual analyte content. A 100 mg/L solution at 98% purity effectively contains 98 mg/L of the active analyte.
4) Why is dilution factor included?
Dilution changes the concentration reaching the detector. The calculator divides the stock concentration by the dilution factor to estimate the corrected concentration during injection.
5) What does injection normalization do?
Injection normalization scales peak area to a common injection volume. It makes runs more comparable when different injection volumes were used during calibration or sample analysis.
6) Can I use this for GC and HPLC methods?
Yes. The calculator is suitable for many GC and HPLC workflows where peak area is proportional to concentration and consistent instrument conditions are maintained.
7) Does recovery correction always need to be used?
No. If your preparation has no extra correction, keep recovery at 100%. Use another value only when you intentionally correct for extraction or analytical recovery.
8) What is the main output for each mode?
The calculator returns either absolute response factor, relative response factor, unknown corrected concentration, or recovered stock concentration, depending on the selected mode.