Measure oligos precisely with sequence-based biology insights. Compare DNA and RNA properties instantly. Export clean reports for labs, classes, and planning.
| Example Sequence | Type | Length (nt) | GC % | Approx. Length (nm) | Use Case |
|---|---|---|---|---|---|
| ATGCGTACCGTTAAGC | DNA | 16 | 50.00% | 5.44 | Short primer design review |
| AUGGCUAACGUU | RNA | 12 | 41.67% | 4.08 | Guide fragment estimate |
| GGGCGCGATATATACC | DNA | 16 | 56.25% | 5.44 | GC-rich oligo screening |
1. Oligo length: Length equals the total count of valid nucleotides in the entered sequence.
2. Physical length: Physical length in nanometers = nucleotide count × base spacing. The default base spacing is 0.34 nm per nucleotide.
3. GC content: GC% = ((G + C) ÷ total valid bases) × 100.
4. Estimated molecular weight: Sum the base weights, then subtract an approximate phosphodiester correction for each linkage.
5. Approximate Tm: For short oligos, the Wallace rule is used. For longer oligos, a common GC-adjusted approximation is applied.
These outputs are screening estimates, not replacements for validated wet-lab conditions or vendor-specific thermodynamic models.
It measures sequence length in nucleotides and estimates related properties such as nanometer length, GC percentage, molecular weight, and approximate melting temperature.
Yes. Select DNA for A, T, G, and C sequences. Select RNA for A, U, G, and C sequences so the calculator validates the correct nucleotide set.
A base spacing near 0.34 nm is commonly used as a practical contour estimate for nucleic acid length calculations. It provides a convenient starting point for quick approximations.
No. The displayed Tm is an approximation for planning and comparison. Salt concentration, mismatches, sequence context, and reaction chemistry can change true melting behavior.
Yes. Any characters outside the selected DNA or RNA base set are ignored. The results are calculated from the valid nucleotides only.
Yes, for quick screening. It is useful for checking size, GC balance, and rough Tm before moving to deeper primer design tools or vendor calculators.
Different nucleotides have different molecular weights. DNA and RNA also differ chemically, so an RNA oligo with the same length can weigh more than DNA.
They are useful for sharing results, keeping lab notes, storing quick sequence summaries, and attaching simple calculation evidence to planning documents.
Important Note: All the Calculators listed in this site are for educational purpose only and we do not guarentee the accuracy of results. Please do consult with other sources as well.