Accurately determine protein concentration fast using advanced chemical parameters now.
The Bradford protein assay is a spectroscopic analytical procedure used to measure the concentration of protein in a solution. Based on the absorbance shift of Coomassie Brilliant Blue G-250 dye under acidic conditions, proteins bind to the dye causing a maximum absorption shift from 465 nm to 595 nm. This straightforward colorimetric test remains a staple in biochemistry laboratories globally for quantifying unknown protein extracts from cellular suspensions, plant tissue homogenates, and recombinant expression systems.
The fundamental computation relies on the linear regression equation derived from your bovine serum albumin (BSA) standard curve: Absorbance = m(Concentration) + c. Rearranging this equation to solve for protein concentration yields:
When analyzing tissue homogenates, additional parameters such as total extraction volume and initial tissue mass help scale the concentration into total yield metrics.
Operating this professional utility requires collecting spectrophotometric data from your assay plates or cuvettes. First, input the raw optical density measured at 595 nm, along with any blank correction values. Second, enter the linear regression parameters (slope and intercept) generated by your standard curve controls. Third, input your sample dilution factor and initial extraction volume parameters. Finally, submit the form to receive instant computations of your target protein metrics.
Important Note: All the Calculators listed in this site are for educational purpose only and we do not guarentee the accuracy of results. Please do consult with other sources as well.