Calculation Result
Calculator Inputs
Stock Solution Dilution
Formula Used
C₁V₁ = C₂V₂
V₁ = (C₂ × V₂) ÷ C₁
Diluent Volume = V₂ − V₁
Dilution Factor = C₁ ÷ C₂
These relationships apply when concentration dimensions are compatible.
How to Use This Calculator
- Select the calculation mode matching your laboratory task.
- Enter concentrations using compatible concentration dimensions.
- Select units for every concentration and volume input.
- Choose decimal precision and number formatting.
- Press Calculate to produce preparation values.
- Review the formula and dilution factor shown.
- Export results using CSV or print to PDF.
Laboratory Dilution Reference Table
| Target Dilution | Stock Fraction | Stock for 100 mL | Diluent for 100 mL | Example Use |
|---|---|---|---|---|
| 1:2 | 0.5 | 50 mL | 50 mL | Two-fold dilution |
| 1:5 | 0.2 | 20 mL | 80 mL | Moderate working dilution |
| 1:10 | 0.1 | 10 mL | 90 mL | Common serial dilution |
| 1:20 | 0.05 | 5 mL | 95 mL | Lower concentration preparation |
| 1:100 | 0.01 | 1 mL | 99 mL | Large dilution factor |
About Laboratory Dilution Calculations
Laboratory dilution calculations help prepare solutions at controlled concentrations. The process commonly starts with a concentrated stock solution. A measured stock volume is transferred into suitable labware. Diluent is then added until the required final volume. The standard relationship is C₁V₁ equals C₂V₂. This relationship works when concentration units describe compatible dimensions. Molar units can convert between M mM µM and nM. Mass concentration units can convert between g/L and mg/mL. Percentage values should remain within the same percentage basis. Mixing molarity with mass concentration requires molecular weight information. This calculator therefore warns against incompatible concentration dimensions.
Stock dilution mode calculates required stock and diluent volumes. Enter the starting concentration and desired working concentration. Then provide the final volume needed for your experiment. The calculator converts compatible units before solving the equation. It also reports the overall dilution factor automatically. Ratio mode handles common preparations such as 1:10 dilutions. A 1:10 dilution contains one part stock overall. The remaining nine parts are the chosen diluent. Percentage mode supports common laboratory percentage preparation planning. Always confirm whether protocols specify w/v v/v or w/w. These percentage systems describe different physical relationships and materials.
Serial dilution mode supports repeated concentration reductions across multiple tubes. Each stage applies the same selected dilution factor. The table reports transferred volume and required diluent volume. It also reports cumulative dilution after every completed step. Serial dilution workflows often support assays and calibration curves. They are also useful for microbiology and analytical testing. Pipetting accuracy becomes increasingly important at very small volumes. Choose volumes appropriate for your available pipette range. Mix each dilution thoroughly before transferring the next aliquot. Use clean tips or suitable contamination controls between transfers. Record stock identity concentration date and preparation details clearly. Verify solution compatibility before using unfamiliar solvents or reagents. This tool supports calculations but cannot validate chemical safety. Follow laboratory procedures and relevant safety documentation during preparation. Careful dilution planning improves accuracy safety and laboratory repeatability.
Frequently Asked Questions
1. What formula does the calculator use?
It primarily uses C₁V₁ = C₂V₂ for compatible solution dilutions.
2. How do I calculate diluent volume?
Subtract the required stock volume from the desired final volume.
3. Can it calculate a 1:10 dilution?
Yes. Ratio mode calculates stock and diluent volumes directly.
4. Does it support serial dilution calculations?
Yes. It creates stepwise concentrations and a preparation table.
5. Can molarity convert to mg/mL automatically?
No. That conversion requires the compound molecular weight.
6. What does dilution factor mean?
It describes how many times the original concentration is reduced.
7. Can I save my laboratory dilution results?
Yes. Download CSV data or print the page as PDF.